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HERDIN Record #: R07-USC-18121510514920 Submitted: 15 December 2018 Modified: 09 January 2019

In vitro antiurolithiatic activity of Onion, Allium cepa var. cepa (L., 1753) bulb extract using spectrophotometric and titrimetric analysis.

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     The incidence of urolithiasis, a condition where urinary supersaturation causes more stone concentrations to form in the urinary tract, continues to ascend across the globe. The current medications employed alleviate only symptoms which poses a need for new formulations addressing urolithiasis. The study, In Vitro Antiurolithiatic Activity of Allium cepa var. cepa focused on the three major steps in crystallogenesis which are nucleation, aggregation and crystal formation. The antiurolithiatic activity of Allium cepa var. cepa was assessed utilizing three assays which are nucleation, aggregation and dissolution assays representing the steps in crytstallogenesis using calcium oxalate crystals to emulate kidney stones. Both nucleation and aggregation assays were executed through spectrophotometric analysis whilst redox titrimetry was employed for dissolution assay. The ethanolic bulb extract of Allium cepa var. cepa was used to assess antiurolithiatic activity and was prepared by maceration. A 100% stock solution of the different plant extract was diluted to 25%, 50% and 75% concentration and subjected to analysis. Out of the different plant concentrations, the 100% test solution showed the highest crystal nucleation inhibition at 54.72%. The same was true for crystal aggregation and crystal dissolution with 73.06% and 93.09%, respectively. A p-value of less than 0.01 was considered significant. The median percent inhibition on crystal nucleation for Allium cepa var. cepa ethanolic extract was 88.09% or 0.88mg/ml. While the median percent inhibition for crystal aggregation was 62.91% or 0.62mg/ml. The effective concentration of Allium cepa var. cepa ethanolic extract for crystal dissolution was 52.72% or 0.53mg/ml. The plant Allium cepa var. cepa was able to act on the all three steps of crystallogenesis - nuclear, aggregation and crystal formation. The extract tested positive for flavonoids, a known antioxidant that prevents crystal formation.

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Pharmacy
Publication Date
March 2018
LocationLocation CodeAvailable FormatAvailability
University of San Carlos-Josef Baumgartner Learning Resource Center, Science and Technology Section T / C97 Fulltext Print Format
1. Abdollah, G , Yazdan, A , Fatemeh, M . "Variation in antioxidant, and antibacterial activities and total phenolic content of the bulbs of mooseer Allium hirtifolium Boiss" ACTAAGRICULTURAESLOVENICA 105(1): 15-22, 2015
2. Agarwal, Kumkum , Ranjana, V . "Ocimungratissimum L: A medicinal plant with promising antiurolithiatic activity" INTERNATIOANL JOURNAL OF PHARMACEUTICAL SCIENCES AND DRUG RESEARCH 6(1): 78-81, 2014
3. Aggarwal, A , Tandon, S , Singla, S K, Tandon, C . "Diminution of oxalate induced renal tubular epithelial cell injury and inhibition of calcium oxalate crystallization in vitro by aqueous extract of Tribuluserrestris" INTERNATIONAL BRAZILIAN JOURNAL OF UROLOGY 36(4): 480-89, 2010
4. Atodariya, Unnati , Roshni, Barad , Upadhyay,, Siddhi , Upadhyay, U . "Anti-urolithiatic activity of Dolichos biflorus seeds" JOURNAL OF PHARMACOGNOSY AND PHYTOCHEMISTRY 2(2): 209-13, 2013
5. Bansode, Prakash , Pankaj, P , Manoj, B . "In vitro urolithiatic activity of Bryophyllum pinnatum against experimentally designed calcium oxalate and calcium phosphate stones" BRITISH JOURNAL OF PHARMACEUTICAL AND MEDICAL RESEARCH 1(1): 34-40, 2016
6. Butterweck, V , Khan, S R. "Herbal medicines in the management of urolithiasis: alternative or complementary?" PLANTA MEDICA 75(10): 1095-1103, 2009
7. Corea, G , Fattorusso, E , Lanzotti, V , Capasso, R , Izzo, A A. "Antispasmodic saponins from bulbs of red onion, Allium cepa L. var. tropea" JOURNAL OFAGRICULTURAL AND FOOD CHEMISTRY 53(4): 935-40, 2005
8. Eldin, I , Taj, E , Ahmed, , AbdElwahab, H M. "Preliminary study of the clinical hypoglycemic effects of Allium cepa (red onion) in type 1 and type 2 diabetic patients" ENVIRONMENTAL HEALTH INSIGHTS 4, 71-77, 2010
9. Eltaweel, M . "Assessment of antimicrobial activity of onion extract (Allium cepa) on Staphylococcus aureus in vitro study." , 2013
10. Finlayson, B , Khan, S R, Hackett, R L. "Mechanisms of stone formation—an overview" SCANNING ELECTRON MICROSCOPY (3): 1419-425, 1984
11. Goldman, I L, Kopelberg, M , Debaene, J E, Schwartz, B S. "Antiplatelet activity in onion (Allium cepa) is sulfur dependent" THROMBOSIS AND HAEMOSTASIS 76(3): 450-452, 1996
12. Kumar, G P. "Evaluation of Tinospora cordifolia for antiurolithiatic potential" JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL SCIENCES 9 (14): 1-5, 2011
13. Gurocak, S , Kupeli, B . "Consumption of historical and current phytotherapeutic agents for urolithiasis:  a critical review" THE JOURNAL OF UROLOGY 176(2): 450-55, 2006
14. Haidari, . "Effects of onion on serum uric acid levels and hepatic xanthine dehydrogenase/xanthine oxidase activities in hyperuricemic rats" Pakistan Journal of Biological Sciences 11(14): 1779-1784, 2008
15. Hannan, A , Humayun, T , Hussain, M B, Yasir, M , Sikandar, S . "In vitro antibacterial activity of onion (Allium cepa) against clinical isolates of Vibrio cholera" JOURNAL OF AYUB MEDICAL COLLEGE 22(2): 160-63, 2010
16. Hess, B , Jordi, S , Zipperle, L , Ettinger, E , Giovanoli, R . "Citrate determines calciumoxalate crystallization kinetics and crystal morphology – studies in the presence of Tomm-Horsfall protein of a healthy subject and a severely recurrent calcium stone former" NEPHROLOGY DIALYSIS TRANSPLANTATION 15(3): 366-374, 2000
17. Hughes, B G, Lawson , L . "Antimicrobial effects of Allium sativum L. (garlic), L. (elephant garlic), and Allium cepa L. (onion), garlic compounds and commercial garlic supplement products" PHYTOTHERAPY RESEARCH 5(4): 154-158, 1991
18. Ige, S F, Salawu, E O, Olaleye, S B, Adeeyo, O A, Badmus, J , Adeleke, A A. "Onion (Allium cepa) extract prevents Cadmium induced renal dysfunction" , 2009
19. Jha, Rahul , Ramani, Pooja , Patel, Dhara , Desai, Sharav , Meshram, Dhananjay . "Phytochemical analysis and in vitro urolithiatic activity of Peltophorumpterocarpum leaves (DC) Baker" JOURNAL OF MEDICINAL PLANTS STUDIES 4(3): 18-22,
20. Prachi, Khare , Kumar, Vinod , Kakkar, Mishra , Arun, NeetuBais , Singh, Rajendra . "Study onin-vitro anti-lithiatic activity of Phyllanthusniruri Linn. leaves by homogenous precipitation and turbiditory method" INTERNATIONAL JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES 6(4): 124-27, 2014
21. Kim, J H. "Anti-bacterial action of onion (Allium cepa L.) extracts against oral pathogenic bacteria" THE JOURNAL OF NIHON UNIVERSITY OF DENTISTRY 39(3): 136-41, 1997
22. Kohjimoto, Y , Ebisuno, M , Tamura, M , Ohkawa, T . "Interactions between calciumoxalate monohydrate crystal and Madin-Darby canine kidney cells: endocytosis and cell proliferation" UROLOGICAL RESEARCH 24(4): 193-199, 1996
23. Lachman, J , Pronek, D , Hejtmankova, A , Dudjak, J , Pivec, V , Faitova, K . "Total polyphenol and main flavonoid antioxidants in different onion (Allium cepa L.) varieties" HORTICULTURAL SCIENCE (PRAGUE) 30(4): 142-47, 2003
24. Lawrence, R , Lawrence, K . "Antioxidant activity of garlic essential oil (Allium sativum) grown in North Indian plains" ASIAN PACIFIC HOURNAL OF TROPICAL BIOMEDICINE 1(1): 51-54, 2001
25. Liakopoulou-Kyriakid, M , Sinakos, Z , Kyriakidis, D A. "Identification of alliin, a constituent of Allium cepa with an inhibitory effect on platelet aggregation" PHYTOCHEMISTRY 24(3): 600-601, 1985
26. Munwar, S , Harekrishna, R . "An in-vitro study on antiurolithiatic activity of ethanolic extract of Catharanthus roseus by titrimetry method" INTERNATIONAL JOURNAL OF LIFE AND BIOSCIENCES 2(2): 125-131, 2016
27. Mustapha, B , Benzeid, H , Idrissi, M.O. B, Larbi, B , Mustapha, D . "In vitro effect of acetylsalicyclic acid on calcium oxalate crystallization: An approach to antilithiasis" INTERNATIONAL JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES 7(3): 329-331, 2015
28. Muthukrishnan, S . "Antiurolithiatic effect of various whole plant extract of Ageratum conzoides Linn. on ethylene glycol induced Urolithiasis in male Wistar albino rats" Int J Pharm Sci Res 5(10): 4499-05, 2014. Retrieved from: http://www.doi.org/10.13040/IJPSR.0975-8232.5(10).4499-05
29. Naseri, M , Kazem, G , Hoda, Y , Arabian, M . "Antispasmodic activity of onion (Allium cepa L.) peel extract on rat ileum" IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH 7(2): 155-159, 2008
30. Naveen, K B, Hindustan, A A, Thippeswamy, M , Sreeramulu, N S, Anuradha, C M, Anil, K K. "Evaluation of diuretic activity with different concentrations of Allium cepa on albino rats" INTERNATIONAL JOURNAL OF CHEMISTRY AND PHARMACEUTICAL SCIENCES 1(1): 56-62, 2012
31. Noorafshan, A , Karbalay-Doust, S , Karimi, F . "Diosmin reduces calcium oxalate deposition and tissue degeneration in nephrolithiasis in rats: a stereological study" KOREAN JOURNAL OF UROLOGY 54(4): 252-257, 2013
32. Ojieh, A E, Adegor, E C, Okolo, A C, Ewhre, L O, Onyesom, I . "Antidiabetic and antilipidemic properties of Allium cepa in streptozotocin-induced diabetes" ENDOCRINE SOCIETY 5(8): 670-671, 2015
33. Saha, S , Verma, R . "Inhibition of calcium oxalate crystallization in vitro by extract of Bergenia ciliate" ARAB JOURNAL OF UROLOGY 11(2): 187-192, 2013
34. Salman, A , Hasan, M , Mahmood, Z . "Antiurolithiatic plants: Formulation used indifferent countries and cultures" PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES 29(6): 2129-2139 , 2016
35. Saravanasingh, K , Ramammurthy, M , Parthiban, P . "In-vitro anti-urolithiatic activity of aerial parts of Aervalanata (L.) Juss" INTERNATIONAL JOURNAL OF CURRENT RESEARCH IN MEDICAL SCIENCES 2(3): 24-27, 2016
36. Sasikala, V , Singanallur, R R, Vijayakumari, B . "In vitro evaluation of Rotula aquatic Lour. for antiurolithiatic activity" JOURNAL OF PHARMACY 6(3): 378-382, 2013
37. Haidari, F , Rashidi, M R, Eshraghian, M R, Mahboob, S A, Shahi, M M, Keshavarz, S A. "Hypouricemic and antioxidant activities of Allium cepaliliaceae and quercetin in normal and hyperuricemic rats" SAUDI MEDICAL JOURNAL 29(11): 1573-1579, 2008
38. Shamsuddeen, S , Bano, R , Shammari, E , Enezi, S . "Risk factors of renal calculi" IOSR JOURNAL OF DENTAL AND MEDICAL SCIENCES 11(6 ): 90-95, 2013
39. Sharma , D , Dey, Y , Sikarwar, I , Sijoria, R , Wanjari, M . "In vitro study of aqueous leaf extract of Chenopodium album for inhibition of calcium oxalate and brushite crystallization" EGYPTIAN JOURNAL OF BASIC AND APPLIED SCIENCES 3(2): 164-171, 2016
40. Thakare, V , Kothavde, P , Dhote, V , Deshpande, A . "Antifertility activity of ethanolic extract of Allium cepa Linn in rats" INTERNATIONAL JOURNAL OF PHARMTECH RESEARCH 1(1): 73-78, 2009
41. Votto, A , Domingues, B , De Souza, M , da Silva Junior, F , Caldas, S , Filgueira, D , Clementin, R , Primal, E , Vallochi, A , Furlong, E , Trindade, G . "Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line" BIOLOGICAL RESEARCH 43(4): 429-437, 2010
42. Katzung, B , Masters, S , Trevor, A . Basic and Clinical Pharmacology.Twelfth Edition McGraw-Hill Companies Inc., Lange Medical Publications, 2012. 151, 154, .
43. Knevel, A , Digangi, F . Jenkins Quantitative Pharmaceutical Chemistry Merriam Webster Bookstore, Inc., 1977.
44. Ross , Ivan . Medicinal plants of the world: Chemical constituents, traditional and modern medicinal uses Totowa, New Jersey: Human Press Inc, 2003.
45. (). Kidney Stones. American Urological Association. . Retrieved from: https://www.auanet.org/education/kidney-stones.cfm
46. Pietrow, Paul , Karellas, Michael . (2005, November 30). American Family Physician. . Retrieved from: http://www.aafp.org/afp/0701/p86.html
47. Santanna, Cristine , Bradike, John , Burnham, Robyn . (2012, November 30). Climbers Censuing Lianas In Mesic Biomes of Easter Regions. . Retrieved from: http://www.climbers.Isa.umich.edu?p=250