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HERDIN Record #: R07-USC-18121009075936 Submitted: 10 December 2018 Modified: 13 December 2018

In vitro thrombolytic activity of the ethanol free peel extracts of Musa Paradisiaca var. Saba (Banana) linn. 1753 using Clot Lysis method.

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                Thrombolytics are used in the management of excessive or blood clot formation. They all work by activating the plasminogen into plasmin which dissolves and degrades the clots. But the major drawbacks with them are prolonged bleeding tendency and anaphylactic reactions. Natural plants and their products with thrombolytic potential and being economical and safe can be considered as alternative for cardiovascular diseases associated with thrombus formation. The purpose of the present study was to assess the thrombolytic activity of Musa paradisiaca var. saba peels using the In-vitro clot lysis assay method. The thrombolytic activity in the ethanol free peel extracts of Musa paradisiacal var. saba peels was determined wherein 20 mg/mL concentration of the peel extract had the highest thrombolytic activity with a value of 0.1655 g and 33.00% in mean weight of clot dissolved and mean percent clot lysis, respectively. Thrombolytic activity could due to the presence of flavonoids, tannins, terpenoids, alkaloids and carbohydrates. Moreover, the results show that the concentration of the peel extract of Musa paradisiacal var. saba is directly proportional and dose dependent because as the concentration increases, its thrombolytic activity also increases.

Publication Type
Thesis Degree
Publication Date
July 2018
LocationLocation CodeAvailable FormatAvailability
University of San Carlos-Josef Baumgartner Learning Resource Center, Science and Technology Section T / AL284 Fulltext Print Format
1. Abbas, K , Rizwani, G H, Zahi, D H, Asif, A . "Pharmacognostic evaluation of Musa paradisiacal L. bract, flower, trachea and tracheal fluid" World Journal of Pharmacy and Pharmaceutical Sciences 4(4): 1461-1475, 2015
2. Adams, D S, Griffi, L A, Nachajko, W R, Reddy, V B, Wei, C M. "A synthetic DNA encoding a modified human urokinase resistant to inhibition by serum plasminogen activator inhibitor" Journal of Biological Chemistry 266, 8476-8482, 1991
3. Akharaiyi, F C, Asoso, O S, Animba, L S. "Antibacterial Activities of Plantain (Musa paradisiacal) Peel and Fruit" Scholars Research Library 8(5): 5-11, 2016
4. Ali, M S, Amin, M R, Kamal, C M, Hossain, M A. "In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of the methanol extract of Adiantum philippense L. Leaves" Asian Journal of Tropical Biomedicine 3(6): 464-469, 2013
5. Ambrosioni, E , Degli, E D. "Thrombolytic drugs in acute myocardial infarct" Cardiologia 12, 395-402, 1991
6. Azad, A K, Islam, O , Rima, E , Islam, E , Sultana, C , Nesa, J U, Ahmed, F . "Phytochemical Screening and in-vitro Thrombolytic Activity of Methanolic Leaf Extract of Zanthoxylum rhetsa" Journal of Pharmaceutical Sciences and Research 7(6): 302-304, 2015
7. Banerjee, A , Christi, Y , Banerjee, U C. "Streptokinase-a clinically useful thrombolytic agent" Research article from Science Direct 22, 287-307, 2003
8. Bhaargavi, V , Sindhuja, S . "Thrombolytic activity of herbal mixture containing aqueous crude extract" International Journal of Research and Development in Pharmacy and Life Science 5(4): 2393-9320, 2016
9. Bhatnagar, S , Garg, V K, Sharma, P K, Jain, S . "Antiulcer activity of Aqueous extract of Musa paradisiacal" Pelagia Research Library 2(4): 40-43, 2011
10. Bhiuya, , et. al., . "In vitro thrombolytic and anti-oxidant activity study of Abroma augusta (Ulatkambal)" International Journal of Science and Technology 14(2): 888-893, 2013
11. Chabuck, Z A, Al-Charrakh, A H, Hindi, N K, Hindi, S K. "Antimicrobial effect of aqueous banana peel extract" Iraq Research Gate Pharm Science 1(73): 5, 2013
12. Chowdhury, K A, Hosen, S Z, Islam, M N, Huq, I , Adnanm Md, , Chy, N U, Kabir, I , Auniq, R B, Uddin, R , Shoibe, M , Chowdhury, M A. "Cytotoxic and thrombolytic activity of roots of Musa paradisiacal (Linn.)." The Pharmacy Innovation Journal 5(8): 97-100, 2017
13. Germain, S , Berdeaux, A , Tissier, R , Ghaleh-Marzban, B , Monnot, C , Galaup, C . "Methods and pharmaceutical compositions for the treatment of ischemic conditions" Institute National de la Santéet de la Recherche (French Institute of Health and Medical Research) , 2016
14. Ghani, A . "Medicinal Plants of Bangladesh: Chemical Constituents and Uses" The Asiatic Society of Bangladesh, Dhaka, Bangladesh 315, 2003
15. Happi, E T, Andrianaivo, R H, Wathelet, B , Tchango, J , Paquot, M . "Effects of the stage of maturation and varieties on the chemical composition of banana and plaintain peels" Food Chemistry 103, 590-600, 2007
16. Imam, M Z, Akhter, S . "Musa paradisiacal L. and Musa sapientum L: A Phytochemical and Pharmacological Review." Journal of Applied Pharmaceutical Science 1(5): 14-20, 2011
17. Jainul, M A, Azam, S , Chowdhury, A , Mamun, M , Rashid, U , Mamun, A A, Chowdhury, H Q. "Evaluation of thrombolytic effect of seven different Bangladesh plants" British Journal of Pharmaceutical Research 4(11): 1400-1406, 2014
18. Jerjes-Sanchez, C . "Venous and arterial thrombosis: A continuous spectrum of the same disease" European Heart Journal 26, 3-4, 2005
19. Kapadia, S P, Pudakalkatti, P S, Shivanaikar, S . "Detection of antimicrobial activity of banana peel (Musa paradisiacal L.) on Porphyromonas gingivalis and Aggregatibacteractinomycete mcomitans: An in vitro study. " Contemporary Clinical Dentistry 6(4): 496-499, 2015
20. Khare, C P. "Indian Medicinal Plants" SpringerScience+BusinessMedia, New York, USA 426, 2007
21. Komorowicz, E , Kolev, K , Lerant, I , Machovich, R . "Flow rate-modulated dissolution of fibrin with clot-embedded and circulating proteases" Circulation Research 10, 1102-8, 1998
22. Kumar, K.S. P, Bhowmik, D , Duraivel , S , Umadevi , M . "Traditional and Medicinal Uses of Banana" Journal of Pharmacognosy and Phytochemistry 1(3): 51-63, 2012
23. Kupchan, S M, Tsou , G , Sigel, C W. "Datiscacin, a novel cytotoxic cucurbitacin 20-acetate from Datisca glomerata" Journal Organic Chemistry 38(7): 1420-1421, 1973
24. Lavanya, K , Abi Beaulah , G , Vani, G . "Musa paradisiacal – A review on Phytochemistry and Pharmacology" World Journal of Pharmaceutical and Medicinal Research 2(6): 163-173, 2016
25. Lijnen , H R, Vanhoef, B , DeCock, F , Okada, K , Ueshima, S , Matsuo, . "On the Mechanism of fibrin-specific plasminogen activation by staphyokinase" Journal of Biological Chemistry 266, 11826-1183, 1991
26. Marder, V J. "Recombinant streptokinase – opportunity for an improved agent" Blood Coagulation Fibrinolysis 4, 1039-1040, 1993
27. Nelson, S C, Ploetz, R C, Kepler, A K. "Musa species (banana and plantain) Research article" Species Profiles Island Agroforestry , 2006. Retrieved from:
28. Nicolini, F A, Nichols, W W, Mehta, J L, Saldeen, T G, Schofield, R , Ross, M , Player, D W, Pohl, G B, Mattsson, C . "Sustained reflow in dogs with coronary thrombosis with K2P, a novel mutant of tissue plasminogen activator" Journal of the American College of Cardiology 20, 228-235, 1992
29. Ogbonna, O A, Izundu, A I, Okoye, N H, Ikeyi, A P. "Phytochemical Compositions of Fruits of Three Musa paradisiacal species at Three Stages of Development" Journal of Pharmacy and Biological Sciences 11(3): 48-59, 2016
30. Panche, A N, Diwan, A D, Chandra, S R. "Flavonoids: an overview" Journal of Nutritional Science 5(47): 1 of 15, 2016
31. Prasad, S , Kashyap, R S, Deopujari, J Y, Purohit, H J, Taori , G M , Daginawala, H F. "Development of an in vitro model to study clot lysis activity of thromolytic drugs" Thromobosis Journal 4(14): 1-4,
32. Ramjan, A , Hossain, M , Runa, J F, Hasanuzzaman, M D, Mahmodul, I . "Evaluation of thrombolytic potential of three medicinal plants available in Bangladesh, as a potent source of thrombolytic compounds" Avicenna Journal of Phytomedicine 4(6): 430-436, 2014
33. Reed, G L, Lui, L , Houng, A K, Matsueda, L H, Lizbeth, H . "Mechanisms of fibrin Independent and fibrin dependent plasminogen activation by streptokinase" Research Circulation , 1998
34. Rutsch, W , Schmutzler, H . "T-PA in thrombolytic therapy of acute myocardial infarct" National Center for Biotechnology Information 6, 336-352, 1994
35. Sakib, M H, Naymul, T , Rana, K , Mohammad, D , Hossain, S , Ullah, S , Mahmood, A A. "In vitro thrombolytic activity of herbal anti-atherosclerosis formulation" European Scientific Journal 11(18): 1857-7881, 2015
36. Saraswathi, N T, Gnanam , F D. "Effect of medicinal plants on the crystallization of cholesterol" J. Cryst. Growth 179, 611-617, 1997
37. Sayeed, M A, Kabir, H , Mamun, M , Rashid, U , Bhuiyan, F A, Rashid, M A. "Thrombolytic activity of methanolic extracts of Desmodium paniculatum (L.) and Sarcochla myspulcherrima (Roxb.)" Bangladesh Pharmaceutical Journal 17(1): 67-69, 2014
38. Shadma, A , Sundaram, S , Rai, G K. "Nutraceutical application and value addition of banana peel: A review" International Journal of Pharmaceutical Sciences and Research 6, 81-5, 2014
39. Shanmugavelu, K G, Rangaswami, G . "Tryptophan and Indole Compounds in Banana Ovaries" Nature , 194-775776, 1962
40. Swathi, D , Jyothi, B , Sravanthi, C . "A Review: Pharmacognostic studies and Pharmacological actions of Musa paradisiacal" International Journal of Innovative Pharmaceutical Research 2(2): 122-125, 2011
41. Uddin, J , Islam, N , Ali, H , Khan, S A, Labu, Z K. "Correlation of Thrombolytic and membrane stabilizing activities with total flavonoid content aerial parts of Spondia pinnata" Bangladesh Pharmaceutical Journal 19(1): 48-52, 2016
42. Valmayor, R V, Jamaluddin, S H, Silayoi, B , Kasumo, S , Danh, L D, Pascua, O C, Espino, R.R. C. "Banana cultivar names and synonyms in Southeast Asia" ,
43. Velumani, S . "Phytochemical and Antioxidant Activity of Banana peel" International Journal of Advance Research and Innovative Ideas in Education 2(1): 91-102, 2016
44. Vestraete, M , Kienast, J . "Pharmacology of the interaction between platelets and vessel wall" 15(2): 493-508, 1985
45. Wu, D H, Shi, G Y, Chuang, W J, Hsu, J M, Young, K C, Chang, C W. "Coiled coil region of streptokinase gamma-domain is essential for plasminogen activation" Journal for Biological Chemistry 276, 15025-1503, 2001
46. Zubair, K L, Samiya, J J, Jala, U , Mostafizur, R . "In vitro investigation of antidhiarrhoeal, antimicrobial and thrombolytic activities of aerial parts of Peperomia pellucida" Pharmacology online 3, 5-13, 2015
47. Marieb, Elaine Nicpon. Essentials of human anatomy and physiology, 10th edition San Francisco CA: Pearson/ Benjamin Cummings, 2012.
48. Stoelting, R V, Dierdof, S F. Handbook for Anesthesia and Co-Existing Disease, 3rd edition 1993.
50. (). Banana varieties. . Retrieved from:
51. Ingelson, K . (). What is thrombus? Causes and types. Medical News Today. . Retrieved from:
52. Klabunde, R . (2014, November 30). Thrombolytic (Fibrinolytic Drugs). Cardivascular Pharmacology Concepts. . Retrieved from:
53. (). Saba banana. . Retrieved from:
54. (). Cardiovascular Diseases (CVDs). World Health Organization. . Retrieved from: