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Submitted: 10 September 2018 Modified: 20 September 2018
HERDIN Record #: R07-USC-18091013232127

In Vitro inhibition of human platelet aggregation of Wachichao (Orthosiphon aristatus Blume, 1858) leaf extract using giemsa microplate assay.

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This study dealt with the inhibition of human platelet aggregation of Wachichao (Orthosiphon aristatus Blume, 1858) Leaf Extract.          


           Methods of extraction were prepared by macerating the leaves of Wachichao O. aristatus Blume with ethyl acetate for 72 hours. Three different test solutions were used with a concentration of 1mg/ml, 2mg/ml, and 4mg/ml labeled as TSA, TSB and TSC, respectively. Normal Saline Solution was used as negative control. Aspirin USP with a concentration of 2mg/ml was used as the positive control. Giemsa Microplate Assay was used in the determination of the inhibition of human platelet aggregation. Three trials were conducted in triplicates.


            Out of the three test solutions, TSC with a concentration of 4mg/ml showed 1 is to 9 to inhibit human platelet aggregation as shown absence of gels using Giemsa Microplate Assay. The result of the wells in TSA (1mg/ml) showed 7 is to 9 and TSB (2mg/ml) showed 4 is to 9 to inhibit human platelet aggregation as shown in the absence of gels in Giemsa microplate assay.


         Thus, TSC having a concentration of 4mg/ml obtained from the leaf extracts of Wachichao (Orthosiphon aristatus Blume, 1858) showed to inhibit human platelet aggregation through the absence of gels in the Giemsa microplate assay. The researcher recommends testing the test groups using spectrophotometry for quantitative analysis. Furthermore, the researcher recommends comparing theresults of Giemsa Microplate Assay and conventional platelet aggregometry (light transmission aggregometry, turbidimetric aggregometry) that measures the in vitro response of platelets to various chemical agents that induce platelet functional responses. Another aspect recommended by the researcher is to compare in vitro study from in vivo.

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Pharmacy
Publication Date
March 2016
LocationLocation CodeAvailable FormatAvailability
University of San Carlos-Josef Baumgartner Learning Resource Center, Science and Technology Section T / M115s Fulltext Print Format (Ask the Information Officer)
1. Alexander, B , Kattlove, K E. "The Effect of Cold Platelets. I. Cold-Induced Platelet Aggregation " Blood 38(1): 39-48, 1971
2. Ahamed, M.B. K., Aisha, A.F. A, Nassar, Z D, Siddiqui, J M, Ismail, Z , Omari, S.M. S, Parish, C R, Majid, A.M.S. A. "Cat" Nutrition and Cancer 64(1): 89-99, 2012
3. Babalola, I T, Shode, F O, Adelakon, E A, Opoku, A R, Mosa, R A. "Platelet Aggregation Inhibitory Activity of Oleanolic Acid, Ursolic Acid, Betulinic Acid and Maslinic Acid" Journal of Pharmacognosy and Phytochemistry 1(6): 54-60, 2013
4. Barcia, J J. "The Giemsa stain: its history and applications " PubMed.Gov 3, 292-6, 2007
5. Chai, T T, Wong, F C, Manan, F A, Ooh, K F, Ismail, N.I. M. "Orthosiphon arisatatus: A review of traditional uses, phytochemical profile, and pharmacologic activities" Traditional and Folk Herbal Medicine 2, 153-187, 2014
6. Damsud, T , Grace, M H, Adisakwattana , S , Phuwapraisirisan, P . "Orthosiphol A from the aerial parts of Orthosiphon aristatus is putatively responsible for hypoglycemic effect of via α-glucosidase inhibition" Natural Product Communications 9(5): 639-641, 2014
7. Guo, C , Lui, S , Guo, Y , Yin, Y , Lin, J , Chen, X , Sun, M . "Comparative Function-Structural Analysis Activities of Flavonoids Phytochemicals" Journal of Animal and Plant Sciences 24(3): 926-935, 2014
8. Guthrie, N , Kurowska, E M, Manthey, J A, Horowitz, R M. "Compositions and Methods of treating, reducing and preventing cardiovascular diseases and disorders with polymethoxyflavones" , 2006
9. Hunafei, D , Reidel, H , Akumo, D N, Gruda, N , Smetanska, I . "Effect of Lactic Acid Bacteria Fermentation on Rosmarinic Acid and Antioxidant Properties of in vitro Shoot Culture of Orthosiphon aristatus as a Model Study" Food Biotechnology 27, 152-177, 2013
10. Lindemann, S , Kramer, B , Seizer, P , Gawaz, M . "Platelets, Inflammation, and Atherosclerosis" Journal of Thrombosis and Haemostasis 5, 203-211, 2007
11. Milton, K . "Factors Influencing Leaf Choice By Howler Monkeys: A Test of Some Hypothesis of Food Selection by Generalist Herbivores " The American Naturalist 114(3): 1979
12. Naghadeh, H T, Badlou, B A, Ferizhandy, A S, Mohammadreza, T S, Shahram, V . "Six hours of resting platelet concentrates stored at 22-24 degree Celsius for 48 Hours in permeable bags preserved pH, swirling, and lactate dehydrogenase better and caused less platelet activation" Blood transfusion 11(3): 400-404, 2013
13. Nayak, A K, Dash, B R, Kar, T , Mandal, K K. "Orthosiphon aristatus (Lamiaceae): A new record for the flora of Odisha, India" Plant Science Reaserch 35, 72-73, 2013
14. Papp, J , Sandor, B , Vamoz, Z , Botor, D , Toth, A , Rabai, M , Kenyeres, P , Cseplo, P , Juricskay, I , Mezosi, E , Koller, A , Toth, K . "Antiplatelet effect acetylsalicylic acid, metamizole and their combination-in vitro and in vivo comparisons" Clinical Hemorheology and Microcirculation 56, 1-12, 2014
15. Pimentel, S.M. V., Bojo, Z P, Roberto, A.V. D, Lazaro, J.E. H, Mangalindan, G C, Florentino, L M, Nazarro, P L, Tasdemir , D , Ireland, C M, Concepcion, G P. "Platelet Aggregation Inhibitors from Philippine Marine Invertebrate Samples Screened in a New Microplate Assay" Marine Biotechnolog 5, 395-400, 2003
16. Shivaprakash, B V, Manjunath, P . "Pharmacology and Clinical Use of Dimethyl Sulfoxide (DMSO): A Review" International Journal of Molecular Veterinary Research 3, 6, 2013
17. Harvey, Richard A. Lippincott United Sates of America: Lippincott-Raven Publishers, 2012. 243-247.
18. Hillyer, C D. Blood banking and transfusion medicine, 2nd edition , 329-330, 2007
19. Liu, Willow J. H.. Traditional Herbal Medicines Research Methods United States of America: John Wiley & Sons, Inc. , 2011. 106.
20. Lao, Felix M. Pharmaceutical Care-Management of Heart Diseases Philippines: Anvil Publishing, 2006. 12.
21. Merrill, Elmer Drew . 1992 An Enumeration of Philippine Flowering Plants London: Forgotten Books, 2013. (Volume 3)
22. Narayanan, Sheshadri , Peerschke, , Ellinor I.B. . Advances in Clinical Chemistry United States of America: Academic Press, 2001. 238.
23. Port, Carol M. Pathology Concepts of Altered Health States, 7th Edition United States of America: Lippincott-Raven Publishers, 2005. 734-737.
24. Manual on the management, maintenance and use of blood cold equipment 2005. 7-8.
25. Wyk, Ben E, Wink, Michael . Medicinal Plants of the Whole World, 1st Edition United State of America: Timber Press, Inc, 2004. 23-25.
26. (). . Retrieved from: http://www.hematology.org/Patients/Basics/
27. Bayalan, H K, Villa, M.P. I, Landicho, D L. (). . Retrieved from: http://philippineculture.ph/filer/toledo-cebu/HERBAL-MEDICINE.pdf
35. Majid, Amin . (). . Retrieved from: http://www.webmedcentral.com/wmcpdf/Article_WMC001361.pdf
36. Mills, Robyn Elizabeth . (). . Retrieved from: http://www.robynelizabethmills.wordpress.com/tag/boats/
38. O, D . (). . Retrieved from: http://anthro.palomar.edu/blood/blood_components.htm
39. Paikin, Jeremey , Eikelboom, John . (). . Retrieved from: http://circ.ahajournals.org/content/125/10/e439.full
40. Ruggeri, Zaverio , Mendolicchio, Loredana . (). . Retrieved from: http://circres.ahajournals.org/content/100/12/1673.abstract
41. Stuart , Godofredo . (). . Retrieved from: http://www.stuartxchange.com/KablingGubat.html
44. Abapo, G. G. I., Nagsuban, E.G. G.. Anti-platelet aggregation activity of the fresh ripe fruits of Muntingia calabura linn. (Manzanitas). 2009. (BS) University of San Carlos,
45. Dela Pena, M.G. T, Dela Pena, J H, Yap, M.G. T. Antiplatelet aggregation activity of the extracts of Carica papaya linn. (Papaya) using giemsa microplate assay. 2008. (BS) University of San Carlos,
46. Diaz, M C, Yana, P.S. A.. Anti-platelet aggregation activity of the extracts of Curcuma longa linn. (Turmeric) Rhizomes. 2009. (BS) University of San Carlos,
47. Sacay, M S, Lachica, E.M. M. Anti-platelet Aggregation Activity of Adiantumcapillus veneralis Linn. (Alambrillo) Extracts. 2010. (BS) University of San Carlos,
48. Tan, M.K. G, Rone, A.L. O. Anti-platelet activity of the extract from leaves of Diplazium esculentum Retz (Pako). 2010. (BS) University of San Carlos,