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Submitted: 09 August 2018 Modified: 12 December 2018
HERDIN Record #: R07-USC-18080911304222

Spatial memory-enhancing activity of Alugbati Basella alba Linn. 1753 (Green var.) leaves and stems on male albino mice using morris water maze.

Bianca Patangan

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                The ability to accurately recall memories is a very important aspect as individuals become older. The intake of certain substances may help with the improvement of memory. Hence, it would be helpful to identify certain plants that are essential for cognitive improvement. The study was done to investigate the possible memory enhancing activity of Basella alba (Alugbati) on male albino mice using the Morris Water Maze method. A standard size pool for mice was used and a platform was placed inside the pool for the mice to find. The leaves and stems of Basella alba were used. Basella alba extract was prepared using soxhlet apparatus with ethanol as the solvent. Upon obtaining the extract, a stock suspension of 3% was formulated using NSS as the solvent and Tween 80 as the suspending agent. The study comprised of three trials, with four mice weighting 25-30g for each trial. For 7 days, the mice were petted for 2 minutes every day. On the 8th day, the training started. The habitualization for output determination lasted for 10 days. After baseline output, three doses of Basella alba, namely 100 mg/kgBW for Group 1, 250 mg/kgBW for Group 2, and 500 mg/kgBW for Group 3, and a negative control (NSS + Tween 80) for Group 4 were then given to the mice to assess retention of memory. Among the different doses, 500 mg/kgBW showed the highest percent time reduction of 65.67%, followed by 250 mg/kgBW with 55.33% and 100 mg/kgBW with 41%. The Percent Time Reduction of all data from the three trials underwent Analysis of Variance (ANOVA) and showed that there is a significant difference among the doses. A Post Hoc analysis (p value<0.01) showed that all doses showed significant difference compared with the negative control, indicating that  all doses exhibit memory enhancing activity. Moreover, results showed that the memory enhancing activity of Alugbati does not depend on the dose given. The researchers would recommend the increase the intake of Alugbati in the diet and the isolation of the constituents of Basella alba to find out which specific constituents cause memory-enhancing activity.

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Pharmacy
Publication Date
March 2014
LocationLocation CodeAvailable FormatAvailability
University of San Carlos-Josef Baumgartner Learning Resource Center, Science and Technology Section T / K576in Fulltext Print Format (Ask the Information Officer)
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