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Submitted: 07 August 2018 Modified: 12 December 2018
HERDIN Record #: R07-USC-18080711504151

In vitro determination of hypoglycemic activity of Mandalusa, Pseuderantemum bicolor (Schrank) Radlk. (1895) leaf extract by inhibition of a-amylase and a-glucosidase enzymes.

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The hypoglycemic activity of Pseuderanthemum bicolor (Schank) Radlk. (1895) leaf extract was determined in vitro using α-amylase and α-glucosidase inhibitory activity assays. The constituents from the dried leaves of P. bicolor were extracted by decoction and maceration with 95% ethanol. Test solutions of 125g/mL, 250g/mL, 500g/mL and 750g/mL from distilled water-free extract, and 25g/mL, 50g/mL, 75g/mL and 100g/mL form ethanol-free extract were prepared for the assays. Inhibitions were measured  based on the absorbance readings of each test solution and the blank. Hypoglycemic activities of the plant extracts were calculated using the percent inhibitions of the test solutions as compared to the positive control, Acarbose. Both the distilled water-free and ethanol-free test solutions were able to exhibit concentration-dependent hypoglycemic activities on     α-glucosidase and α-amylase. The percent inhibition on α-glucosidase increased as the concentration of the test solutions increased. The half maximal inhibitory concentrations (IC50) for the α-glucosidase inhibitory activity assay were 664.12g/mL for distilled water-free solution and 63.11g/mL for ethanol-free soulution. The ethanol-free test solution with 100g/mL concentration exhibited the highest hypoglycemic activity on α-glucosidase, at 215.18±8.07%, which can be  correlated on the presence of alkaloids and phenols in the ethanol-free extract. On the other hand, the percent inhibition on α-amylase decreased as the concentration of the test solutions increased. The distilled water-free test solutions with 125g/mL concentration posted the highest hypoglycemic activity at 38.52±2.54%, which can be attributed to the presence of phenols and saponins in the distilled water-free extract. However, the presence of starch-hydrolyzing saponins and reducing sugar in the plant could also have affected the absorbance reading of 3-amino-5nitrosalicylic acid in the alpha-amylase inhibitory activity assay.

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Pharmacy
Publication Date
October 2014
LocationLocation CodeAvailable FormatAvailability
University of San Carlos-Josef Baumgartner Learning Resource Center, Science and Technology Section T / C278m Fulltext Print Format (Ask the Information Officer)
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