Report an error   

HERDIN Record #: R07-USC-18080416153599 Submitted: 05 August 2018 Modified: 12 December 2018

In vitro hypoglycemic activity of Blumea balsamifera Linn. 1833 (Gabon) leaf extract by inhibition of a-amylase and a-glucosidase enzymes.

See More

The hypoglycemic activity of the Blumea balsamifera Linn. 1833 (Gabon) leaf extract was determined by α-amylase and α-glucosidase inhibitory activity assay. Three solvents were used for the extraction; distilled water for decoction, 95% ethanol, and dichloromethane for maceration. The absorbance of the by-products in α-amylase and α-glucosidase assay are 3-amino-5-nitrosalicylic  acid, a red-orange solution was measured at 540nm, and p-nitrophenol, a yellow solution, was  measured at 540nm, p-nitrophenol, a yellow solution, was measured at 405nm respectively. Decrease in concentration resulted to decrease in absorbance, making the percent (%) inhibition and percent (%) hypoglycemic activity increased. For α-amylase assay with 0.075mg/mL concentration, distilled water-free test solution has the  highest percent hypoglycemic activity at 97.48%, followed by ethanol-free test solution at 72.49% then dichloromethane-free test solution  at 54.82%. For α-glucosidase assay, results showed that with a concentration of 0.075mg/mL, distilled water-free test solution has the highest percent hypoglycemic activity at 95.66%, followed by ethanol-free test solution at 71.49% then dichloromethane-free test solution at 51.48%. The hypoglycemic activity of  the test extract of B. balsamifera could possibly be due to the presence of saponins, tannins, phenolic compounds and terpenes. The researchers recommend that further investigation be done on the isolation, purification and characterization of the chemical constituents specifically total phenolic content and saponins which are responsible for the hypoglycemic activity found in the leaves of Blumea balsamifera. It is also recommended that in α-amylase assay, the temperature used in boiling the test tubes must be constant in all extracts to compare the activity per extract. It is also recommended for a new method of assay and enzymes other than α-amylase and α-glucosidase such as lipase, sucrose and glucoamylase to further find out if the test extracts of B. balsamifera will exhibit hypoglycemic activity.

Publication Type
Thesis Degree
Publication Date
October 2014
LocationLocation CodeAvailable FormatAvailability
University of San Carlos-Josef Baumgartner Learning Resource Center, Science and Technology Section T / Es61k Fulltext Print Format
1. Senthikumar, Annadurai , Kannathasan, Krishnan , Vankatesalu, Venugopalan . "Chemical constituents and larvicidal property of the essential oil of Blue mollis (D. Don) Merr. against Culex Quinquefasciatus." Parasitol Res 103, 959-962, 2008. Retrieved from: http://10.1007/s00436-008-1085-2
2. Kumar Ashok, BS , Lakshaman, K , Jayaveera, K N, Sheshadri, Shekar D, Narayan Swamy, V B, Saleemula, Khan , Velumurga, C . "In Vitro a-Amylase Inhibition and Antioxidant Activities on Methanolic Extract of Amaranthus Caudatus Linn Oman" Medical Journal 3, 166-170, 2011
3. Lee, Dong Gu , Mok, So-Youn , Choi, Changsun , Cho, Eun Ju , Kim, Hyun Young , Lee, Sanghyun . "Analysis of apigenin in Blumea balsamifera Linn DC and its inhibitory activity agains aldose reductase in rat lens" Journal of Agricultural Chemistry and Environment , 28-33, 2012
4. Thevenod, Frank . "Pathophysiology of Diabetes Mellitus Type 2: Roles of Obesity, Insulin Resistance and B-Cell Dysfunction. " Epidemiological Evidence and Molecular Links Front Diabetes 19, 1-18, 2008
5. Arungarinathan, Ganesan , McKay, Gerard A, Fisher, Miles . "Drugs for diabetes: part 4 acarbose." Br J Cardiol 18(2): 78-81, 2011
6. Kubota, Hiroaki , Kojima-Yuasa, Akiko , Morii, Risako , Huang, Xuedan , Toshio, Norikura , Rho, Sook-Nyung . "Anti-Obesity Effects of Blumea balsamifera Extract in 3T3-L1 Preadipocytes and Adipocytes" Am. J. Chin. Med. 37, 843, 2009. Retrieved from: http://10.1142/S0192415X09007326
7. Lodge, J A, Maier, T , Liebl, W , Hoffman, , Strater, N . "Crystal structure of Thermotoga maritima alpha-glucosidase AglA defines a new clan of NAD+ dependent glycosidases" J. Biol. Chem. 278, 19151-1915, 2003
8. Kalyan, Roy , Saha, Shubhankar , Biswas, Subhasis , Ahmed, Wasif , Mariappan, G . "In vivo Assessment of Antidiabetic and Antioxidant Activities of Blumea balsamifera in Streptozotocin-diabetic Rats" Research Journal of Medicinal Plant 7, 48-57, 2013
9. Zhu, Liang , Tian, Ying-Juan , Yang, Li , Jiang, Gou-Jian . "Chemical Composition and antimicrobial activities of essential oil of Blumea megacephala" EXCLI Journal 10, 62-68, 2011
10. Narkhede, M B, Ajimire, P V, Wagh, A E, Manoj, Mohan , Shivashanmugam, AT . "In vitro antidiabetic activity of Caesalpina digyna (R.) methanol root extract" Asian Journal of Plant Science and Research 1(2): 101-106, 2011
11. Rahman, Mohammad Habibur , Islam, Zakirul , Miya, Faruk , Munshi, Mita , Nishi, Afroza Rahman , Nur Alam, S.M. , Hasan, Nazmul . "Study on Hypoglycemic Activity of Methanolic Extract of Blumea lacera (Local Name: Bonomula) leaves on swiss albino mice" In proceeding of: El XXII Congreso de la Sociedad Italo Latinoamericana de Etnomedicina 1, 2013
12. Owolabi, M S, Lajide, L , VIllanueva, H E. "Essential oil composition and insecticidal activity of Blumea perrottetiana growing in southwestern Nigeria" Nat Prod Commun 5(7): 1135-8, 2010
13. Pednekar, Prajakta P., Vakil, Babu V., Sane, Ramesh T., Datar, Ajit G.. "Antimicrobial Activity of essential oil of Blumea Eriantha DC against skin pathogens." Journal of Pharmacy and Pharmaceutical Sciences , 2012
14. de Sales, Paloma Michelle , Monteiro de Souza, Paula , Simeoni, Luiz Alberto , de Oliveira Magalhae, Perola , Silveira, Damaris . "a-Amylase Inhibitors: A review of Raw Material and Isolated Compounds from Plant Source" J Pharm Pharmaceut Sci 15(1): 141-183, 2011. Retrieved from:
15. Nair, Sindhu S., Kavrekar, Vaibhavi , Mishra, Anshu . "In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts" European Journal of Experimental Biology 3(1): 128-132, 2013
16. Paul, Santa , Devi, Nilakshee . "Floral morphology and foliar anatomy of Blumea lanceolaria (Roxburgh) Druce [Asteraceae]" Pleione 7(1): 32-38, 2013
17. Sakee, U , Maneerat, S , Cushnie, T P, De-Eknamkul, W . "Antimicrobial activity of Blumea balsamifera (Lin.) DC. extracts and essential oil" Nat Prod Res. 19, 1849-56, 2011. Retrieved from: http://10.1080/14786419.2010.485573
18. Kumar, Sunil , Narwal, Smita , Prakash, Om . "a-glucosidase inhibitors from plants: A natural approach to treat diabetes" Pharmacognosy Reviews 5(9): 19-29, 2011
19. Premkumar, Thatan , Lee, Kyungjae , Geckeler, Kurt E. "Shape-tailoring and catalytic function of anisotropic gold nanostructures" Nanoscale Research Letters 6(1): 547, 2011
20. Norikura, Toshio , Kojima-Yuasa, Akiko , Shimizu, Miki , Huang, Xuedan , Xu, Shenghui , Kametani, Saeda , Rho, Sook-Nyung , Kennedy, David Opare, Matsui-Yuasa, Isao . "Anticancer activities and mechanisms of Blumea balsamifera extract in hepatocellular carcinoma cells" The American Journal of Chinese Medicine 36(2): 411-24, 2008. Retrieved from: http://10.1142/S0192415X08005862
21. Singh, Umesh Pratap , Sing, Arvind Kumar , Sarathy, Partha . "Effect of methanolic extracts of Blumea eriantha DC leaves on protein metabolism and marker enzymes in streptozotocin-induced hyperglycemic animals" International Journal of Pharmacy and Pharmaceutical Sciences 4, 2011
22. Gardner, David G., Shoback, Dolores . Greenspan New York: McGraw-Hill Medical, 2011. Chapter 7.
23. Gutierrez, Hermes . Philippine Materia Medica 184-185. (Volume 1)
24. Enzymatic Assay of a-GLUCOSIDASE (EC p-Nitrophenyl a-D-Glucoside as Substrate SIGMA:
25. Taiwanese Native Medicinal Plants Phytopharmacology and Therapeutic Values Taylor & Francis Group, 2006.
26. Yousefi, Fatemeh , Mahjoub, Soleiman , Pouramir, Mahdi , Khadir, Fatemeh . "Hypoglycemic activity of Pyrus biossieriana Buhse leaf extract and arbutin: Inhibitory effects on alpha amylase and alpha glucosidase" , 2013
27. Goodsell, D . "Alpha-amylase" , 2006. Retrieved from:
28. (2011, November 30). ActiveOneHealth. . Retrieved from:
29. (2013, November 30). International Diabetes Foundation. . Retrieved from:
30. (2012, November 30). Laboratories Servier Diabetes (Type 2). . Retrieved from:
31. (2010, November 30). Blumea balsamifera Linn DC. Philippine Medicinal Plants. . Retrieved from:
32. (2010, November 30). Biochemistry 253 Human alpha-amylase. RCSB. . Retrieved from:
33. (2010, November 30). US Food and Drug Administration. . Retrieved from:
34. (2013, November 30). World Health Organization. . Retrieved from:
37. (2010, November 30). Why they don. . Retrieved from: