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Submitted: 31 July 2018 Modified: 12 December 2018
HERDIN Record #: R07-USC-18073111033722

The antimutagenic activity of Labanos Raphanus sativus L. var. longipinnatus vegetable juice on male albino mice.

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Cancer is the third leading cause of mortality in the country and studies have shown that people who consume a diet high in fruits and vegetables reduce their task of cancer. Radish, a crucifer, has gained prominence in the field of cancer prevention because it contains many beneficial phytochemicals. Labanos, the Filipino radish, is an annual plant widely cultivated in the Philippines at all altitudes. In this study, Labanos is tested a functional food  and as a potent phytomedicine to prevent cancer by studying its antimutagenic activity. The antimutagenic activity of Labanos was examined through as in vivo micronucleus assay of the Labanos in treatment-free test juice form. The Micronucleus assay detects freshly-induced structural chromosomal damages in bone marrow cells and administration of the Labanos test juice is tested for the ability to lower the incidence of induced micronuclei which serve as the index for genetic damage. Mutation was induced through intraperitoneal administration Mitomycin C. The microncuclei  Labanos  juice was tested using four doses: 1000 mg (Group A), 500 mg (Group B), 250 mg (Group C), and 150 mg (Group D) doses per 20g body weight (BW). The untreated group (Group E) was not administered with the test juice while the negative control (Group F) was given water injection. The mice in Group A developed an average of 81 Micronucleated Polychromatic Erythrocytes (MPCEs) per 1000 Polychromatic Erythrocytes (PCEs) translating to a 69.99% reduction of MPCEs. As the dosage further decreased, there was a respective decrease in the percent reduction thus showing a linear, dose-dependent antimutagenic activity. This linear relationship is confirmed by a correlation coefficient of (+) 0.886. The one-way analysis of variance (p> 0.01) and post-hoc analysis showed that all variables have significant differences. The results suggest that the Labanos test juice is antimutagenic, with an ED50 (Median Effective Dose) of 510mg. Phytochemicals established to be natural antimutagenic agents are saponins and phenolics, both of which were found in the Labanos test juice in the phytochemical screening through the test tube method. The researchers recommend the characterization and quantitative analysis of total phenolic and saponnin content in Labanos.

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Pharmacy
Publication Date
March 2014
LocationLocation CodeAvailable FormatAvailability
University of San Carlos-Josef Baumgartner Learning Resource Center, Science and Technology Section T / Ec75g Fulltext Print Format (Ask the Information Officer)
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