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Submitted: 30 July 2018 Modified: 12 December 2018
HERDIN Record #: R07-USC-18073013541444

Adaptogenic assessment of Vegetable Fern "Pako" Diplazium esculentum (Retz., 1803) leaves on male albino mice using swim stress method.

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"Pako" or vegetable fern is widely distributed in the Philippines and has been used for quite some time as an alternative in treating various illnesses. Swimming has been widely used for studying the physiological changes and the capacity of small laboratory animals in response to stress. Male albino mice were used as test animals. Three treatment groups were treated with the test drug extract in three different doses (250 mg/1000g, 100mg/1000g and 50mg/1000g) for seven days by oral route. The fourth group served as a negative control group and was treated with distilled water with Tween 80 for seven days by oral route. The other members of these groups were also treated with their respective treatment plans for the second and third trials in the same manner. Untreated group of test animals were given the standard pellet diet and water ad libitum but no treatment was given. The results showed that the stock solution of the ethanol-free crude Diplazium esculentum extract with the different dose of 250mg/1000g, 100mg/1000g and 50mg/1000g showed adaptogenic activity by reducing the increase of blood glucose and cholesterol levels after the swim stress test. The researchers suggest: the measurement of the baseline parameters in order to find out if there is already a pre-existing decrease in the said biochemical parameters  so as to find out if the Diplazium esculentum extract has hypoglycemic and/or antihyperlipidemic activity; the use of rats in order to allow treatment plans of higher doses; the addition of more trials; addition of other biochemical parameters and a longer timeframe of treatment.

Publication Type
Thesis Degree
Publication Date
October 2014
LocationLocation CodeAvailable FormatAvailability
University of San Carlos-Josef Baumgartner Learning Resource Center, Science and Technology Section T / C124al Fulltext Print Format (Ask the Information Officer)
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