Report an error   


Submitted: 24 October 2014 Modified: 27 October 2014
HERDIN Record #: R07-USC-14102406345039

Determination of the antibacterial activity of the tannin extract from fresh leaves of Psidium guajava linn. (Bayabas)

See More

Normal 0 false false false EN-US X-NONE X-NONE


/* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin-top:0in; mso-para-margin-right:0in; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0in; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;}


This research dealt with the determination of the antibacterial activity of the tannin extract from fresh guava leaves. Decoction was employed in the preparation of extractive and was saturated with sodium chloride (table salt) to obtain tannin constituent. The tannin isolate was made into 250 mh/ml test to solution. Gentamicin Sensi Disc 10 ug was used as the protective control. Distilled water was the negative control.


The antibacterial activity was determined by Kirby Bauer Disc Diffusion Method using three test organisms namely Escherichia coli, Psedomonas aeruginosa and Staphylococcus aureus. After sixteen hours of incubation, the zone produced by the positive control, negative control  and test solution was measured.


The 250 mg/ml and 500 mg/ml test solution showed antibacterial activity against Staphylococcus aureus but no antibacterial activity to Escherichia coli and Pseudomonas aeruginosa. The 500 mg/ml test solution showed a larger antibacterial activity against Staphylococcus aureus. The percent antibacterial activity was 41.05% in trial 1, 38.57% in trial 2 and 36.76%in trial 3. While the 250 mg/ml percent antibacterial activity was 37.51% in trial 1, 31.41% in trial 2 and 30.89% in trial 3 against Staphylococcus.


The 250 mg/ml and 500 mg/ml produced no zone of inhibition, indicating that two solution did not inhibit the growth of both Escherichia coli and Staphylococcus aureus.


The researchers recommends to test the tannin to other microorganism. A statistical treatment should be done to determine the significance of the antibacterial activity of the plant.

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Pharmacy
Publication Date
March 2007

Objectives

The goal of the study is to determine the antibacterial activity of tannins from Psidium guajava Linn.


Specifically, this study sought to:


1. determine the diameter of zone of inhibition around the disk impregnated with the tannin extract from Psidium guajava linn.


2. identify which bacteria (Escherichia coli, Staphylococcus aureus or Pseudomonas aureginosa) was most inhibited by the tannin extract.

LocationLocation CodeAvailable FormatAvailability
University of San Carlos-Josef Baumgartner Learning Resource Center, Science and Technology Section T/G985m Abstract Print Format
1. Baron, J. E., Finegold, M. S.. Diagnostic microbiolgy. 1996.
2. Black, J. E., Creager, G. J.. Microbiology: principles and applications. 1990.
3. Boyd, R. F., Hoerl, R. G.. Basic medical microbiology. 1986.
4. Chambers, H. F.. General principles of antimicrobial therapy, Goodman and Gilaman. 2006.
5. Elardo, S. M.. Microbiology and parasitology laboratory workbook.
6. Evans, C. E.. Trease and Evans: pharmacognosy. 2002.
7. Genarro, R. A.. Remington: the science and practice of pharmacy. 2000.
8. Quisumbing, E. . Medicinal plants of the Philippines. 1978.
9. Tortora, G. J., Case, C. L.. Microbiology: an introduction. 1986.
10. Tyler, V. E., Brady, L. R.. Pharmacognosy.
12. (). . Retrieved from: http://www.answers.com/gentamicin&r=67
13. (). . Retrieved from: http://www.jupar.org
19. Lee, C. A., Cantiller, P. C.. "Antibiogram of nosocomial staphylococcal isolates from philippine general hospital in the first quarter of 2001" 51, 21-29,
20. Valdez, S. , Cadulada, J. . "Drug resistance of E. coli isolates from selected chicken and workers in two poultry farms" 51, 1-8,
21. Puglisi, M. , Engel, S. . "Antimicrobial activities of extracts from Indo-pacific marine plants against marine pathogens and saprophytes" 150(4):
22. Alpuerto, M. D., Daclan, J. . The antibacterial activity of Mimosa pudica linn ( Makahiya) leaf extract against Escherichia coli and S. aureus. 2003. (BS) University of San Carlos,
23. Chu, D. . Qualitative and quantitative analysis of the tannin content of the hot water extract of the Stem Bark of Psidium guajava linn. 1988. (BS) University of San Carlos,
24. Eng, J., E., Uy, L. A.. A comparative study on the antibacterial activity of tannins from the fresh leaves of guava, attapulgite and loperamide. 2001. (BS) University of San Carlos,
25. Evangelista, C. , Yap, S. . The antibacterial activity of tannin extract from carmona retusa vatil matam (Tsaang Gubat) leaves. 2003. (BS) University of San Carlos,
26. Isnani, S. L., Palanca, C. . Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the tannin solution isolated from mature green leaves of talisai. 2005. (BS) University of San Carlos,
27. Sardane, A. , Lumapas, C. . The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determination of the partially purified tannins from the fresh leaves of Spondias purpurea linn. (sineguelas). 2005. (BS) University of San Carlos,
28. Subla, K. , Lumayag, J. , Lumayag, J. . The antibacterial activity of fresh leaves of Heliotropium indicum linn. (elepante). 2003. (BS) University of San Carlos,
29. Tan, L. , Otud, M. J.. The antibacterial activity of the extracts from the leaves of Muntingia calabura linn. (Mansanitas). 2003. (BS) University of San Carlos,

Citation