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Submitted: 10 July 2014 Modified: 24 October 2014
HERDIN Record #: R07-USC-14071009223320

Antibacterial potential of the metabolites of marine sponge-associated microorganisms

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Sponges are renowned to have rich biologically active metabolites, many of which have antimicrobial, antiviral or antitumor properties. In this study, five different metabolites of marine sponge-associated microorganisms, coded as MPR 1-1, MPR 1-8, MPR 5-5, MPR 5-6 and MPR 6-3, were used. By using the streak plate method, the five different marine sponge-associated growth of colonies. A loopful of each marine sponge-associated microorganism was inoculated into ten mL M1 broth and was shaken for two consecutive days. After the two-day shaking, the bacterial inoculums were upscaled separately to produce more metabolites by adding the ten mL broth to a 300 mL M1 broth in a 500 mL Erlenmeyer flask. These were again shaken for two consecutive days. Equal amounts of methanol were added to each of the M1 broth culture to allow cell lysis, this releasing the metabolites. Kirby-Bauer Disk Diffusion Method was used to determine the antibacterial activity of the metabolites against the test microorganisms. Out of the five metabolites, with a stock concentration of 20 microliters per disk, MPR 1-1, MPR 1-8, MPR 5-5 and MPR 6-3 exhibited an antibacterial activity against Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. MPR 1-1 gave an average zone of inhibition of 21.91 mm in Staphylococcus aureus, 10 mm in Escherichia coli and 13.98 mm in Bacillus subtilis. MPR 1-8 gave an average zone of inhibition of 20.99 mm in Staphylococcus aureus, 8.11 mm in Escherichia coli and 11.97 in Bacillus subtilis. MPR 5-5 gave an average zone of inhibition of 22.87 mm in Staphylococcus aureus, 9.11 in Escherichia coli and 19.98 mm in Bacillus subtilis. MPR 5-6 gave an average zone of inhibition of 19.01 mm in Staphylococcus aureus and 11.22 mm on Bacillus subtilis. It did not show any zone of inhibition of Escherichia coli. Lastly, MPR 6-3 gave an average zone of inhibition of 22.65 mm in Staphylococcus aureus, 10.22 mm in Escherichia coli and 17.1 m in Bacillus subtilis. All of the test solutions did not show any antibacterial activity of Psuedomonas aeruginosa. The most susceptible test microorganism to all the test solution is Staphylococcus aureus. The test solution that gave a strong antibacterial activity against the most susceptible test microorganism is test solution test C (MPR 5-5). The researcher recommends that the antibacterial potential of the five test solutions would be further tested using of thin layer chromatography (TLC). It also recommended that these test solutions be subjected to Fourier Transform Infrared (FTIR) Spectroscopy to determine the functional groups present on the test solution used. The researchers would also recommend the use of GC-MS for the identification of the specific constituents responsible for the antibacterial activity.

Publication Type
Thesis Degree
Publication Date
March 2007
LocationLocation CodeAvailable FormatAvailability
University of San Carlos-Josef Baumgartner Learning Resource Center, Science and Technology Section T/R33r Abstract Print Format
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