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Submitted: 23 June 2014 Modified: 10 October 2014
HERDIN Record #: R07-USC-14062317184395

Antiplatelet aggregation activity of the extracts of Carica papaya linn. (Papaya) using giemsa microplate assay


The study dealt on the quantitative analysis of the ant platelet aggregation activity of the extracts of Carica papaya Linn. Using the Giemsa Microplate Assay. The method used in obtaining the test extracts was maceration with 95% ethanol and further separation followed through solvent - partitioning with hexane and ethyl acetate, and finally thorough removal of solvent using eppendorf vacufuge. The test Solutions A. (Ethanol- free Extract), B (Hexane- free Extract), and C (Ethyl Acetate-free Extract) used were prepared by dissolving the dried solvent - free extracts in Normal Saline Solution (NSS) to obtain different concentrations of 100mg/mL, 250mg/mL and 500 mg/mL of each test solution. Aspirin (10mg/5mL) was used as the positive control. NSS was used as the negative control and 0.25M calcium chloride was used as an inducer of platelet aggregation. Platelet suspension from human blood type O positive was used and was suspended in a microtitre plate with incubation at 37?C. The test solutions and aspirin were added to the platelet suspension. Calcium chloride was likewise added to induce aggregation of platelets. Giemsa dye was added to each well for easy visualization of the gels on the microplate. Each well was observed for the presence of violet gels to indicate platelet aggregation response. There were nine test solutions used in the study. The extracts from the ethanol extractive of Carica papya Linn. Namely test solution B2 (250 mg/mL) and test Solution B3 (500mg/mL) were able to inhibit platelet aggregation relative to the positive control, Aspirin. The test did not able to inhibit the platelet aggregation. The use of one-day old platelet suspension for further study, the use f the crude extracts to be tested for the activity, for further study, the use of the crude extract of the papaya leaves is recommended. The use of other parts of the plant like the fruit sap which may contain constituents that have antiplatelet activity is also rtecommended.

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Pharmacy
Publication Date
March 2008
LocationLocation CodeAvailable FormatAvailability
University of San Carlos - Josef Baumgartner Learning Resource Center, Science and Technology Section T/D37j Abstract Print Format
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