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HERDIN Record #: R07-USC-14052815063389 Submitted: 28 May 2014 Modified: 15 October 2014

Anti-platelet aggregation activity of the fresh ripe fruits of Muntingia calabura linn. (manzanitas)

This study dealt with the anti-platelet aggregation activity of fresh ripe fruit of Muntingia calabura Linn. (mazanitas). Manzinatas are known to have antiseptic, astringent and antispasmodic properties. Study also shows bioactivity of manzanitas which includes antibacterial property. Fresh ripe fruits of manzanitas were used in the study. Methods of extraction included expression and masceration. Five different test solutions with 100mg/mL concentrations were used. There were five test solutions used in the Giemsa Micoplate Assay, test solution 1 (Pure Manzanitas Juice), test solution 2 (CrudeEthanol-free), test solution 3 (Hexane-free), test solution 4 (Ethyl acatate-free) and test solution 5 (Ethanol-free). Aspirin solution with 2 mg/mL concentration and dimethyl sulfoxide (DMSO) were used as positive and negative controls, respectively. Giemsa Microplate assay was used in the determination of the anti-platelet aggregation activity. Three trials were conducted in quadraplicates. The test determination of the anti-platelet aggregation was subjected to phytochemical screening using thin layer chromatography. Out of the five test solutions , only test solution 5 (Ethanol-free) inhibited platelet aggregation at concentration of 100mg/mL as shown by the absence of violet gels using the Giesma Microplate Assay. Test solution 1-4 did not inhibit platelet aggregation. In the phytochemical screening, it was found out that the ethanol-free extract contain flavonoids and tannins. The ripe fruits of manzanitas had anti-platelet aggregation activity using the Giesma Microplate Assay. It is recommended that all the test solutions must undergo phytochemical screening to determine the active constituents present. Cell counting of the aggregated platelets should also be done in order to have analysis of the anti-platelet.

Publication Type
Thesis Degree
Publication Date
October 2009
LocationLocation CodeAvailable FormatAvailability
University of San Carlos - Josef Baumgartner Learning Resource Center, Science and Technology Section T/Ab17g Abstract Print Format
1. Chen, J. J., Lee, H. H.. "New dihydrochalcones and anti-platelet aggregation constituents from the leaves of Muntingia calabura linn." 73(6): 572-577,
2. Mekhfi, H. , ElHaouari, M. . "Effects of extracts and tannins from Arbutus unedo leaves on rat platelet aggregation" 20(2): 135-139,
3. Pimentel, S. V., Bojo, Z. P.. "Platelet aggregation inhibitors from philippine marine invertebrate samples screened in a new microplate assay" 5(4): 395-400,
4. Plotnikov, M. B., Smolyakova, V. I.. "Antithrombotic and antiplatelet activity of ortho-isobornyl phenol derivative" 145(3): 328-330,
5. Allison, R. , Anderson, S. C.. Clinical hermatology: principles, procedures and correlations.
6. Armstrong, E. J.. Principles of pharmacology.
7. Brunton, L. , Lazo, J. S.. Goodman.
8. Digangi, F. E., Knevel, A. . Jenkin.
9. Howland, R. D., Mycek, M. . Pharmacology.
10. Guevara, B. . A guidebook to plant screening: phytochemical and biological.
11. Mckenry, L. M.. Mosby.
12. Zdanowicz, M. M.. Essentials of pathophysiology for pharmacy.
13. Quisumbing, E. . Medicinal plants of the Philippines.
14. (). . Retrieved from:
15. (). . Retrieved from:
16. (). . Retrieved from:
24. Miro, M. M., Relatorres, J. L.. Antiplatelet aggregation activity of the bulbs of Allium sativum linn. (garlic) using the Giemsa microplate assay. (BS) University of San Carlos, 2007
25. Otud, M. J., Tan, L. . The antibacterial activity of the extracts of fresh leaves of Muntingia calabura linn. (manzanitas). (BS) University of San Carlos, 2003
26. Elardo, S.M. P.. Protocol of common in vivo and in vitro bench-top asssays. 2003