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HERDIN Record #: R07-USC-13071812115521 Submitted: 18 July 2013 Modified: 13 September 2013

Antimutagenic activity of the leaf extract of cebu cinnamon cinnamomum cebuense (kostermans, 1986)

This study dealt with the determination of the antimutagenic activity of the most bioactive test solution of Cinnamomum cebuense (Cebu cinnamon).
The most bioactive test solution was determined through the brine shrimp lethality assay. The test solution prepared from the crude extract stock solution proved to be the most bioactive test solution. The antimutagenic activity was assessed using in vitromicronucleus test on albino mice. Doses of 1000mg, 500mg and 250mg and 150mg per 20g mouse were tested. Mitomycin C was used to induce the information of macronucleated polychromatic erythrocytes (MPCEs) in the bone marrow cells of the test animals. Water for the injection was used as the negative control. Test solutions and the negative control were administered orally 10minutes after the intraperitoneal administration of Mitomycin C, with the dose of 3mcg per 0.20 mL. Oral administration of the second dose of the test solution was done 24 hours after the administration of the first dose.
Result showed that the crude extract test solutions of Cebu cinnamon reduce the formation of MPCEs induced by Mitomycin C indicating antimutagenic activity of all four test solution doses: 77.61% for the 1000mg/20g dose, 67.76% for the 500mg/20g dose, 46.85% for the 250mg/20g dose and 23.62% for the 150mg/20g dose.
Use of other methods such as REC assay, Ames Test and Host-mediated Assay are recommended for further studies of the antimutagenic activity of Cebu cinnamon. Futhermore, isolation and characterization of the constituents responsible for the antimutagenic activity is highly recommended.

Publication Type
Thesis Degree
Publication Date
October 2011


This study aimed to determine the antimutagenic activity of the extract from the leaves of Cinnamomum cebuense (Cebu cinnamon) using micronucleus test on male albino mice.
Specifically, this study aimed to:
1. determine and compare the calculated Lethal Concentration 50 (LC50) of the different test solutions using the brine shrimp lethality assay;
2. determine the antimutagenic activity of the most bioactive (toxic) test solution using the Micronucleus Test.

LocationLocation CodeAvailable FormatAvailability
University of San Carlos - JB LRC, Science and Technology T/C818sh Abstract Print Format
1. Ko, A. , Dollinger, M. , Rosenbaum, E. . Everyone's guide to cancer therapy: how cancer is diagnosed, treated and managed day to day.
2. Rahman, A. . Techniques for drug development.
3. Sylianco, C. . Genetic toxicity.
4. Del Fierro, R. S.. Studies on the mutagenecity, clastogenecity and antimutagenecity potential of tinospora rumphii boerlage.
5. Maquilang, Q. A.. Clastogenecity and antimutagenecity potential of Oregano, Coleus amboinicus Loureiro.
6. Ribu, R. . Isolation and characterization of antimutagen for Kalatsusi leaves.
7. Sialana, F. J.. The cytotoxic and antimutagenic metabolite of the MActan MArine Sponge: its isolation and characterization.
10. (). . Retrieved from:
14. Abo-Zeid, , Mona, A.M. , Farghaly, A. A.. "The anti-mutagenic activity of piperine against mitomycin C induced Sister chromatid exchanges and Chromosomal abberations in Mice" 7(12): 72-78,
15. Anderson, J. E., Goetz, C.M. , McLaughlin, J. L.. "A blind comparison of simple bench-top bioassay and human tumour cell cytotoxicities as antitumor prescreens" 2(3): 107-111,
16. Edenharder, R. , van Petersdorf, I. . "Antimutagenic effects of flavonoids, chalcones and structurally related compounds on the activity of 2-amino and 3-methylimidazole quinoline and other heterocyclic amine mutagens from cooked food" 287, 261-274,
17. Gawish, A.M. , Ramadan, S. , Hassan, A.M. . "Morphometrical Histopathological and cytogenetical ameliorating effectsof Green Tea Extract on Nicotine toxicity of the testis of Rats" 1, 105,
18. Kada, T. , Kaneko, K. , Matsuzaki, T. . "Detection and chemical identification of natural bio-antimutagens" 150, 127-132,
19. Maridass, M. . "Evaluation of Brine Shrimp Lethality of Cinnamomum species" ,
20. Meyer, B. N., Ferrigni, N. R.. "Brine Shrimp: a convenient general bioassay for active plant constituents" 45, 31-34,
21. Moshi, M. J., Innocent, E. , Masimba, P. J.. "Antimicrobial and brine shrimp toxicity of some plants used in traditional medicine in Bukoba District, North Western Tanzania" 11(1): 23-28,
22. Nakasugi, T. , Komai, K. . "Antimutagens in the Barsilian folk medicinal plant carqueja" 46, 2560-2564,
23. Opoku, A. R., Geheeb-Keller, M. , Lin, J. . "Preliminary screening of some traditional Zulu medicinal plants for antineoplastic activities versus the HepG2 cell line" 14(7): 534-537,
24. Rojanapo, W. , Tepsuwan, A. . "Antimutagenic and mutagenic potentials of Chinese Radish environmental health perspectives supplement" 101(3): 247-252,
25. Sarkar, D. , Sharma, A. . "Plant extracts as modulators of genotoxic effects" 62, 275-300,
26. Scheck, A.C. , Perry, K. , Hank, N. C., Clark, W. D.. "Anticancer activity of extracts derived from the mature roots of Scutellaria baicalensis on human malignant brain tumor cells" 6, 27,
27. Sumanth, M. , Chowdary, G. N.. "Antimutagenic activity of aqueous extract of Momordica charantia." 1(4): 42-46,
28. Yen, G. C., Chen, H.Y. . "Relationship between antimutagenic activty and major components of various teas" 11, 37-41,