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Submitted: 18 July 2013 Modified: 13 September 2013
HERDIN Record #: R07-USC-13071812115521

Antimutagenic activity of the leaf extract of cebu cinnamon cinnamomum cebuense (kostermans, 1986)


This study dealt with the determination of the antimutagenic activity of the most bioactive test solution of Cinnamomum cebuense (Cebu cinnamon).
The most bioactive test solution was determined through the brine shrimp lethality assay. The test solution prepared from the crude extract stock solution proved to be the most bioactive test solution. The antimutagenic activity was assessed using in vitromicronucleus test on albino mice. Doses of 1000mg, 500mg and 250mg and 150mg per 20g mouse were tested. Mitomycin C was used to induce the information of macronucleated polychromatic erythrocytes (MPCEs) in the bone marrow cells of the test animals. Water for the injection was used as the negative control. Test solutions and the negative control were administered orally 10minutes after the intraperitoneal administration of Mitomycin C, with the dose of 3mcg per 0.20 mL. Oral administration of the second dose of the test solution was done 24 hours after the administration of the first dose.
Result showed that the crude extract test solutions of Cebu cinnamon reduce the formation of MPCEs induced by Mitomycin C indicating antimutagenic activity of all four test solution doses: 77.61% for the 1000mg/20g dose, 67.76% for the 500mg/20g dose, 46.85% for the 250mg/20g dose and 23.62% for the 150mg/20g dose.
Use of other methods such as REC assay, Ames Test and Host-mediated Assay are recommended for further studies of the antimutagenic activity of Cebu cinnamon. Futhermore, isolation and characterization of the constituents responsible for the antimutagenic activity is highly recommended.

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Pharmacy
Publication Date
October 2011

Objectives

This study aimed to determine the antimutagenic activity of the extract from the leaves of Cinnamomum cebuense (Cebu cinnamon) using micronucleus test on male albino mice.
Specifically, this study aimed to:
1. determine and compare the calculated Lethal Concentration 50 (LC50) of the different test solutions using the brine shrimp lethality assay;
2. determine the antimutagenic activity of the most bioactive (toxic) test solution using the Micronucleus Test.

LocationLocation CodeAvailable FormatAvailability
University of San Carlos - JB LRC, Science and Technology T/C818sh Abstract Print Format
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