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Submitted: 16 July 2013 Modified: 05 September 2015
HERDIN Record #: R07-USC-13071609274272

Analgesic activity of the crude extract from the dried leaves of gmelina arborea roxb. (paper tree) on male albino mice

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The dried leaves of Gmelima arborea Roxb. was subjected to the test for the presence of analgesic action, using the Hot Plate Method. The method of extraction was maceration of the dried leaves with 95% ethanol for 3 days. The extract collected was allowed to dry. Five grams of the dried extract was dissolved in 10 mL of distilled water, which served as the stock solution for the 100mg/20g dose and 300mg/20g dose of the test solution. A total of 3 trials were performed with 3 replicates per trial and 3 mice per replicate (30, 60 and 90 minutes after administration). Male albino mice weighing 25-30 grams were used for analgesic testing by showing a delayed paw shaking, licking of paws and jumping of the mice after placing the mice on the hot plate. Ibuprofen, at a concentration of 200mg/50mL and distilled water were used as a positive control and negative control, respectively. The test solution of 100mg/20g dose and 300mg/20g dose exhibited an analgesic activity. The 100mg/20g dose of the test solution exhibited a %MPA of 26.06%, 32.18% and 38.24% at 30, 60 and 90 minutes after oral administration, respectively. The 300mg/20g dose of the test solution exhibited a %MPA OF 23.91%, 31.69% and 37.10% at 30, 60 and 90 minutes after oral administration, respectively. The positive control exhibited a %MPA of 51.07%, 52.48% and 50.55% at 30, 60 and 90 minutes after oral administration, respectively. A p-value of 1.63E-33 was obtained between the time intervals after oral administration of the test solution, which indicates a significant difference. A p-value of 8.86E-94 was obtained between the positive control, negative control and the test solution which indicates a significant difference between the analgesic activity of the different test solutions. This proves the presence of analgesic activity of Gmelina arborea Roxb. extract, however, the positive control remained to have the highest analgesic activity.

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Pharmacy
Publication Date
October 2011

Objectives

In this study, the researchers aimed to determine the analgesic activity of the crude extract from the dried leaves of Paper Tree (Gmelina arborea Roxb.) on male albino mice. Specifically, it aimed to determine the following: 1. The analgesic action of the crude extract from the dried leaves of Paper Tree (Gmelina arborea Roxb.) by the comparison of the % Maximum Possible Analgesic of the different groups. 2. The onset of analgesic action of the crude extract from the dried leaves of Paper Tree (Gmelina arborea Roxb.) based on 30, 60 and 90 minutes after oral administration.

LocationLocation CodeAvailable FormatAvailability
University of San Carlos-Josef Baumgartner Learning Resource Center, Science and Technology Section T/T56ia Abstract Print Format
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